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Diagnostics

HPV Test: Which test truly provides an answer?

The Dutch standard test only tells you if you have HPV. But not which type, not how much virus, and not whether it is getting worse. A better test exists.

The problem with the Dutch standard test

There are two main HPV tests used by gynecologists and general practitioners (GPs). In the Netherlands, the Aptima test is primarily used. This test gives a simple answer: positive or negative. The test detects a mix of high-risk HPV types all at once, without specifying which type you have.

Dutch test vs. Belgian test

HPV Test Comparison

Feature Dutch test (Aptima) Belgian test (RIATOL qPCR)
Result Positive or negative Quantitative viral DNA per type
Genotyping None (mix of types) 18 individual HPV types
Viral load Not measured Exact amount of virus
Genes detected mRNA E6/E7 oncogenes per type
Monitoring possible No Yes, viral load progression
Experience Since NL screening program 15+ years routinely in Belgium
Technology mRNA detection Multiplex PCR

The Belgian RIATOL qPCR test has been used routinely in Belgium for over 15 years. It is a multiplex PCR test that measures quantitative viral DNA and individually detects the E6/E7 oncogenes of 18 different HPV types. PCR technology is very specific and sensitive: it also picks up low concentrations of the virus.

The same smear test, two completely different results

To make the difference concrete: the same smear test can lead to completely different results in the Netherlands and Belgium.

Case study: same smear test, two laboratories

Dutch lab Belgian lab
Smear test PAP 1 PAP 1
HPV result HPV negative HPV 59 present in <1.0 in the cells
Consequence Return to 5-yearly screening program Monitoring and follow-up

In this case, HPV 59 was not picked up by the Dutch test, while the Belgian lab did detect the virus. The consequence is significant: based on the Dutch result, this woman would not be tested again for another 5 years. In that interval, the viral load can increase and abnormal cells can develop.

Example of a quantitative HPV test lab result with individual HPV types
Steady increase

of the viral load precedes the development of CIN 3+. By monitoring the viral load, you can signal a deterioration earlier.

Why genotyping and viral load matter

The quantitative HPV test offers two crucial advantages over the standard test:

1. Genotyping: which HPV type do you have?

Not every HPV type is equally dangerous. HPV 16 and 18 are the most aggressive types and are responsible for the majority of cervical cancer cases. Knowing which type you have helps determine the risk and the urgency of treatment.

2. Viral load: how much virus is present?

The amount of virus in your cells is an important indicator. A steady increase in viral load precedes the development of severe abnormalities (CIN 3+). By regularly measuring the viral load, you can track the progression and intervene in a timely manner.

Graph: HPV 59 viral load over time, measured in copies per cell

If HPV is not detected while it is actually present, you may be wrongly sent back to the 5-yearly screening program. During those years, the virus can continue unhindered. This is a risk you can avoid with the right test.

The HPV test via HPV Health

At HPV Health, we work with the quantitative PCR test. This provides us and you with the information needed to create a well-informed treatment plan:

  • Exactly which HPV type you have (out of 18 types)
  • How much virus is present (viral load)
  • The ability to monitor progress with follow-up measurements

Based on these results, we create an individual treatment plan focused on strengthening your immune system and targeted management of the infection.

Do you want to know which HPV type you have and how much virus is present?

The quantitative HPV test gives you the information you need. No more vague 'positive' results, but concrete data we can work with together.

Request the HPV test

Veelgestelde vragen

Are the tests reliable?
The reliability of an HPV test depends on several factors. These include the quality of the sample material, the timing of the test, and the way the laboratory performs the analysis. A correctly performed test in a certified laboratory can be very reliable, but errors in sampling or timing can affect the result. The specificity of HPV tests in the Netherlands is high, at 96% for CIN 2 and CIN 3.
Can I also have the quantitative test done through my general practitioner (GP)?
In most cases, no. The Aptima test is the standard in the Netherlands, and GPs usually do not have access to the Belgian PCR test. You can have this test performed through HPV Health.
How often should I repeat the HPV test?
This depends on your situation. With an active HPV infection, we generally advise a follow-up test after 3 to 6 months to monitor the progression of the viral load. We adjust the treatment plan based on the results.
What if my Dutch test is negative but I still have symptoms?
A negative result on the Dutch standard test does not completely rule out the presence of HPV. The Aptima test can miss low concentrations of the virus. If you have symptoms or an abnormal smear test, a quantitative PCR test may still detect HPV.
Are the costs of the test reimbursed?
The quantitative HPV test falls outside the standard reimbursement package of health insurers. The costs are at your own expense. Contact us for current rates.

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